0974-4150 (Online)
0974-4169 (Print)

Author(s): M.L.N. Acharyulu, P.V.S.R. Mohana Rao, I. Siva Ramakoti, P. Jayarangarao


DOI: 10.5958/0974-4150.2021.00004.3   

Address: M.L.N. Acharyulu1, P.V.S.R. Mohana Rao2, I. Siva Ramakoti3, P. Jayarangarao4
1Associate Professor, Centurion University of Technology and Management, Andhra Pradesh- 530017, India
2Research Scholar, Department of Engineering Chemistry, A.U. College of Engineering (A), Visakhapatnam - 530003, A.P
3Assistant Professor, Department of Chemistry, Centurion University of Technology and Management, Odisha- 761211, India
4Assistant Professor, Baba Institute of Technology and Sciences, Visakhapatnam, A.P. India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 1,     Year - 2021

Two visible spectrophotometric methods were developed Aand B for the determination of Darunavir in pure and pharmaceutical formulations. The methods are based on condensation reaction with PDAB (Method-A) and ONB (Method-B) in presence of acidic medium with the primary amine group in DNV. The coloured products exhibit absorption ?max at 639 nm and 452nm for methods A and B respectively. Regression analysis of Beer-Lambert plots showed good correlation in the concentration ranges 10-60µg/ml, 50-300µg/ml, correlation co-efficients are 0.9983, 0.9989; Sandell’s sensitivities are 9.9833 x 10-3, 3.0456 x 10-2 (1 mole cm-1); and molar absorptivity values are 5.4857 x 104, 1.7981x 104 (µg cm¬-2) for methods-Aand B respectively. The proposed methods are applied to commercial available formulations and the results are statistically compared with those obtained by the UV reference method and validated by recovery studies. The results are found satisfactory and reproducible. These methods are applied successfully for the estimation of the DNV in the presence of other ingredients that are usually present in formulations. These methods offer the advantages of rapidity, simplicity and sensitivity and low cost without the need for expensive instrumentation and reagents.

Cite this article:
M.L.N. Acharyulu, P.V.S.R. Mohana Rao, I. Siva Ramakoti, P. Jayarangarao. Condensation Methods for the Determination of Methoxamine Hydro Chloride in Pure and Pharmaceutical Formulations. Asian J. Research Chem. 2021; 14(1):23-27. doi: 10.5958/0974-4150.2021.00004.3

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