Author(s):
Avinash Mahadeo Bhagwat, Pratiksha Vitthal Awaghade, Atisha Jayprakash Katte, Nishigandha Dayanand Naikawadi
Email(s):
avinashbhagwat2167@gmail.com
DOI:
10.52711/0974-4150.2024.00034 
Address:
Avinash Mahadeo Bhagwat*, Pratiksha Vitthal Awaghade, Atisha Jayprakash Katte, Nishigandha Dayanand Naikawadi
Department of B. Pharmacy, YSPM’s Yashoda Technical Campus, Faculty of Pharmacy, Wadhe, Satara 415 011.
*Corresponding Author
Published In:
Volume - 17,
Issue - 4,
Year - 2024
ABSTRACT:
Febuxostat is a selective inhibitor of xanthine oxidase that is used to treat hyperuricemia in gout patients. For the determination of Febuxostat, an isocratic liquid chromatographic technique RP-HPLC was devised and validated. By using phosphate buffer (pH 3.0): methanol: acetonitrile (40:20:40, v/v) as a Mobile phase and 1.0 mL/min flow rate at room temperature, chromatographic separation was obtained on a (4.6 x 150mm 3.5um Agilent XBD) C18 column. The injection capacity is 20 liters, and the run time is 7 minutes. The wavelength of detection is set to 317nm. The linear correlation coefficient (R2) was found to be 1.000, with a -0.0035 percent y intercept. The detection limit was determined to be 0.0056ppm while the quantification limit was found to be 0.018ppm. Febuxostat was proven to be 99.85% pure. The requirements for repeatability and precision have been met. For the determination of Febuxostat in pharmaceutical dosage forms, the approach is simple, specific, precise, durable, and accurate (tablets).
Cite this article:
Avinash Mahadeo Bhagwat, Pratiksha Vitthal Awaghade, Atisha Jayprakash Katte, Nishigandha Dayanand Naikawadi. A New Isocratic RP-HPLC Assay Method Development and Validation of Febuxostat in Tablet Dosage Form. Asian Journal of Research in Chemistry. 2024; 17(4):187-4. doi: 10.52711/0974-4150.2024.00034
Cite(Electronic):
Avinash Mahadeo Bhagwat, Pratiksha Vitthal Awaghade, Atisha Jayprakash Katte, Nishigandha Dayanand Naikawadi. A New Isocratic RP-HPLC Assay Method Development and Validation of Febuxostat in Tablet Dosage Form. Asian Journal of Research in Chemistry. 2024; 17(4):187-4. doi: 10.52711/0974-4150.2024.00034 Available on: https://ajrconline.org/AbstractView.aspx?PID=2024-17-4-1
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