ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Cijo John, C. Chainulu, Priyankar Ghosh, Seema Srivastava, Sudhirkumar Shukla, S. Satyanarayana

Email(s): cijojohn01@gmail.com

DOI: Not Available

Address: Cijo John1*, C. Chainulu1, Priyankar Ghosh1, Seema Srivastava1, Sudhirkumar Shukla2 and S. Satyanarayana3
1Central Forensic Science Laboratory, Govt. of India, Hyderabad, India- 500 013
2Central Forensic Science Laboratory, Govt. of India, Chandigarh, India-160 036
3Department of Chemistry, Osmania University, Hyderabad, India- 500 007
*Corresponding Author

Published In:   Volume - 4,      Issue - 7,     Year - 2011


ABSTRACT:
A new simple, precise, rapid and selective High-Performance Thin-Layer Chromatographic (HPTLC) method has been developed for the simultaneous determination of amitriptyline (AMTRL), atenolol (ATL) and propranolol (PRPL) using benzene: methanol: acetone: ammonia solution (25% NH3) (5:3:2:0.2; v:v) as the mobile phase and Merck HPTLC plates (0.2 mm thickness) pre coated with 60F254 silica gel on aluminium sheet as the stationary phase. Detection was carried out densitometrically using a UV detector at 254 nm. The retention factors (Rf value) of AMTRL, ATL and PRPL were 0.80, 0.37 and 0.69 respectively. The developed method was fully validated as per the guidelines given by the International conference on Harmonisation (ICH) for precision, recovery, accuracy, robustness, ruggedness and specificity and determination of the limits of detection and quantification. The amount of AMTR, PRPL and ATL present in marketed tablets was quantified and estimated using the developed method which was found to be in close agreement with the labelled content. The linear regression analysis for calibration plots showed good linear relationship in the concentration range of 0.1ug/spot to 10ug/spot with respect to peak area.


Cite this article:
Cijo John, C. Chainulu, Priyankar Ghosh, Seema Srivastava, Sudhirkumar Shukla, S. Satyanarayana. Validated High Performance Thin Layer Chromatography Method for the Simultaneous Determination of Amitriptyline, Atenolol and Propranolol. Asian J. Research Chem. 4(7): July, 2011; Page 1059-1063.


Recomonded Articles:

Author(s): Vatchavai Bhaskara Raju, Bonthu Mohan Gandhi, Kamatham Srinivas Sumanth, Kolli Srinivas, Tupakula N Venkata Lakshmi Neeraja

DOI: 10.5958/0974-4150.2017.00030.X         Access: Open Access Read More

Author(s): SS Chitlange, Kiran Bagri, DM Sakarkar

DOI:         Access: Open Access Read More

Author(s): Ismail, R Rajavel, M Ganesh, M Jagadeeswaran, K Srinivasan, J Valarmathi, T Sivakumar

DOI:         Access: Open Access Read More

Author(s): VB Kshirsagar, UA Deokate, VB Bharkad, SS Khadabadi

DOI:         Access: Open Access Read More

Author(s): Sachin R Shinde , Suvarna I Bhoir, Namdev S Pawar, Suman B Yadav, Ajay S Ghumatkar, Ashok M Bhagwat

DOI:         Access: Open Access Read More

Author(s): Sravani A

DOI: 10.5958/0974-4150.2018.00144.X         Access: Open Access Read More

Author(s): Tentu Nageswara Rao, Muralidhar Reddy Avuthu, B. Venkata Reddy, SNVS Murthy

DOI: 10.5958/0974-4150.2016.00076.6         Access: Open Access Read More

Author(s): Prasanna Reddy Battu, MS Reddy

DOI:         Access: Open Access Read More

Author(s): Babu Ganesan, Perumal Perumal, Vijaya Baskaran Manickam, Surya Rao Srikakolapu, Srujana Divya Gotteti, Latha Sundaresan Thirumurthy

DOI:         Access: Open Access Read More

Author(s): Nilesh Jain, Ruchi Jain, Hemant Swami, Deepak Kumar Jain

DOI:         Access: Open Access Read More

Author(s): Mohd. Idris, S.N. Rasool, K.M. Varshney, S.K. Shukla, T.R. Baggi

DOI:         Access: Open Access Read More

Author(s): Wankhede SB, Prakash A, Chitlange SS

DOI:         Access: Open Access Read More

Author(s): Sunil Singh, Nitin Dubey, D.K. Jain

DOI:         Access: Open Access Read More

Author(s): Kathiravan S., Anbarasi B., Mathankumar S.

DOI:         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles


Recent Articles




Tags