Sejal K. Patel, Harshil R. Patel
Sejal K. Patel, Harshil R. Patel*
Department of Quality Assurance, S.K. Patel College of Pharmaceutical Education & Research, Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
Volume - 6,
Issue - 4,
Year - 2013
A simple, accurate, and rapid reversed phase high performance liquid chromatographic method has been developed and validated for the simultaneous estimation of Eperisone Hydrochloride (EPE) and Lornoxicam (LOR) in synthetic mixture. The separation was carried out using mobile phase consisting of methanol: ACN: water (60: 30: 10, v/v/v) (pH-3, adjusted with OPA). The column used was Phenomenex C18, (250 mm x 4.6 mm i.d., 5 µm) with flow rate 1 ml/min using PDA detection at 255 nm. The method was linear over a concentration range 10 – 100 µg/ml for EPE and 2 – 20 µg/ml for LOR. The retention time of Eperisone hydrochloride and Lornoxicam were found to be 2.2 min and 3.15 min respectively. Results of analysis were validated statistically and by recovery studies. The mean recovery was 99.07 ± 0.42 and 100.79 ± 1.05 for EPE and LOR respectively. The limit of detection (LOD) and the limit of quantification (LOQ) for EPE and LOR were found to be 0.3504 and 1.0618 µg/ml and 0.1903 and 0.5769 µg/ml, respectively. The results of the study showed that the proposed RP-HPLC method was found to be simple, sensitive, precise and accurate and also useful for the routine determination of EPE and LOR in mixture.
Cite this article:
Sejal K. Patel, Harshil R. Patel. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Eperisone Hydrochloride and Lornoxicam in Synthetic Mixture. Asian J. Research Chem. 6(4): April 2013; Page 372-376.
Sejal K. Patel, Harshil R. Patel. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Eperisone Hydrochloride and Lornoxicam in Synthetic Mixture. Asian J. Research Chem. 6(4): April 2013; Page 372-376. Available on: https://ajrconline.org/AbstractView.aspx?PID=2013-6-4-12