ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): K. Vijaya Sri, K. Prajawala, S. Deepthi, K. Niharika

Email(s): vijayasree_2002@yahoo.co.in

DOI: 10.5958/0974-4150.2018.00129.3   

Address: K. Vijaya Sri*, K. Prajawala, S. Deepthi, K. Niharika
Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, (Affiliated to Osmania University) Maisammaguda, Secunderabad-500 014
*Corresponding Author

Published In:   Volume - 11,      Issue - 4,     Year - 2018


ABSTRACT:
A simple, selective, rapid, precise and accurate for the UV and High performance liquid chromatographic method for determination of capecitabine in bulk and its pharmaceutical formulations. Same solvent used as UV and RP-HPLC method. The mobile phase consisting mixture of acetonitrile: water 0.1% ortho phosphoric acid (50:50) v/v. Eclipse C18 column (5 µm particle size x 4.6 × 250 mm) was used as a stationary phase and flow rate for 0.8ml/min. UV and HPLC analysis of capecitabine was carried out at a wavelength of 242nm. The linear regression analysis data for the calibration curve showed a good linear relationship with a correlation coefficient of UV/HPLC 0.999 and 0.998. The UV and HPLC of linear regression equation was y=0.167X-0.024 and .In HPLC method sharp peak of capecitabine at a retention time of 1.67min.Validation parameters were evaluated for the method according to the ICH (Q2R1) guidelines. The limit of detection and limit of quantification for the UV and HPLC method were 0.103 and 0.312 0.042 and 0.021 respectively. The %RSD values for Intra-day precision and Inter-day precision were found to be 0.31 % and 0.30% respectively. Accuracy of the method was determined through recovery studies which were found to be within 99-102.22% for both the methods. In HPLC method precision, accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is better than the earlier methods developed for the quantification of capecitabine in bulk and pharmaceutical dosage form.


Cite this article:
K. Vijaya Sri, K. Prajawala, S. Deepthi, K. Niharika. Method Development and Validation of UV and RP-HPLC method for the estimation of Capecitabine in Bulk and Pharmaceutical Dosage Forms. Asian J. Research Chem. 2018; 11(4):731-738. doi: 10.5958/0974-4150.2018.00129.3

Cite(Electronic):
K. Vijaya Sri, K. Prajawala, S. Deepthi, K. Niharika. Method Development and Validation of UV and RP-HPLC method for the estimation of Capecitabine in Bulk and Pharmaceutical Dosage Forms. Asian J. Research Chem. 2018; 11(4):731-738. doi: 10.5958/0974-4150.2018.00129.3   Available on: https://ajrconline.org/AbstractView.aspx?PID=2018-11-4-7


Recomonded Articles:

Author(s): Bulusu Lakshmi Sushma1, Gutta Madhusudhan, Anireddy Jayashree

DOI: 10.5958/0974-4150.2017.00014.1         Access: Open Access Read More

Author(s): Y. Padmavathi, Akari Anjali, Nayaka Raghavendra Babu, P Ravi Kumar

DOI: 10.5958/0974-4150.2017.00064.5         Access: Open Access Read More

Author(s): Vatchavai Bhaskara Raju, Bonthu Mohan Gandhi, Kamatham Srinivas Sumanth, Kolli Srinivas, Tupakula N Venkata Lakshmi Neeraja

DOI: 10.5958/0974-4150.2017.00030.X         Access: Open Access Read More

Author(s): Alka Verma, Bhupesh Verma, Sunil Kumar Prajapati, Kishu Tripathi

DOI:         Access: Open Access Read More

Author(s): SS Chitlange, Kiran Bagri, DM Sakarkar

DOI:         Access: Open Access Read More

Author(s): Mukund Nagarnaik, Arun Sarjoshi, Ajay Bodkhe, Bhupendra Khanal, Mayuri Pise, Girish Pandya

DOI: 10.5958/0974-4150.2015.00102.9         Access: Open Access Read More

Author(s): Banerjee S, Bonde CG, Merukar SS, Patil YR

DOI:         Access: Open Access Read More

Author(s): Kadhiravansivasamy, S. Sivajiganesan, T. Periyathambi, V. Nandhakumar, M. Pugazhenthi

DOI: 10.5958/0974-4150.2017.00016.5         Access: Open Access Read More

Author(s): Tentu Nageswara Rao, Muralidhar Reddy Avuthu, B. Venkata Reddy, SNVS Murthy

DOI: 10.5958/0974-4150.2016.00076.6         Access: Open Access Read More

Author(s): B. Balaswami, P. Venkata Ramana, B. Subba Rao, P. Sanjeeva

DOI: 10.5958/0974-4150.2018.00023.8         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles


Recent Articles




Tags