ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): Imene Guediri, Chérifa Boubekri, Ouanissa Smara, Touhami Lanez

Email(s): cherifa.boubekri@univ-biskra.dz , imanguediri20@gmail , ouanissasm@yahoo.fr , touhami-lanez@univ-eloued.dz

DOI: 10.5958/0974-4150.2021.00008.0   

Address: Imene Guediri1,4, Chérifa Boubekri2,4*, Ouanissa Smara1,5 and Touhami Lanez3,4
1University Kasdi Merbah, Faculty of Material Science, Department of Chemistry, Ghardaia BP Road 511. 30,000, Ouargla, Algeria
2*University Mohamed Khider, Faculty of Exact sciences, Natural and Lile Sciences, Department of Material sciences, BP145 RP Biskra 07000 Algeria.
3University of Echahid Hamma Lakhdar, Faculty of Exact sciences, PO Box 789, 39000, El Oued, Algeria
4University of El Oued, VTRS Laboratory, B.P.789, 39000, El Oued, Algeria
5Laboratory Valorisation et Promotion des Ressources Sahariennes (VPRS), 30000, Ouargla, Algeria
*Corresponding Author

Published In:   Volume - 14,      Issue - 1,     Year - 2021


ABSTRACT:
In the present research, we studied the quantification of the total phenolics, total flavonoids, total flavonols, and the evaluation of the antioxidant activity of the fruits of Solanum nigrum L. by chemical and electrochemical methods. The chloroform, ethyl acetate, and n-butanol crude extracts are prepared from the hydroethanolic extract of the fruits were tested. The results showed that ethyl acetate extract showed the highest phenolic content (87.68mg GAE/g), chloroform extract showed the highest flavonoid content (24.23mg QE/g) and highest flavonol content (71.60mg RE/g). The evaluation of antioxidant activity by chemical methods revealed that the highest DPPH radical scavenging activity was shown by ethyl acetate extract (0.123mg/mL) while the highest ferric reducing capacity was shown by butanol extract (610.46±0.0015mg FeSO4/g). The electrochemical study of the three extracts by cyclic voltammetry method indicates that the highest antioxidant activity was shown by ethyl acetate extract (17.75±0.238mg/g) equivalent ascorbic acid. The superoxide scavenging assay of chloroform extract showed the highest value (0.8 mg/mL).


Cite this article:
Imene Guediri, Chérifa Boubekri, Ouanissa Smara, Touhami Lanez. Total phenolic contents and determination of Antioxidant activity by DPPH, FRAP, and cyclic voltammetry of the fruit of Solanum nigrum (black nightshade) growing in the south of Algeria. Asian J. Research Chem. 2021; 14(1):47-55. doi: 10.5958/0974-4150.2021.00008.0


REFERENCE:
1.    Shlini P, Siddalinga Murthy KR. Extraction of Phenolics, Proteins and Antioxidant Activity from Defatted Tamarind Kernel Powder. Asian J. Research Chem. 2011; 4(6): 936-941.
2.    Datir SB, Patel AM, Patel AK, Patil PP, Rohit FI, Thorat VD, Bharti DK, Ganjare AB. Evaluation of Antioxidant Activity of the Aerial Parts of the Abutilon indicum (Linn) Sweet (Malvaceae). Research J. Pharmacology and Pharmacodynamics. 2010; 2(5): 324-327.
3.    Subedi L, Timalsena S, Duwadi P, Thapa R, Paudel A and Parajuli K. Antioxidant activity and phenol and flavonoid contents of eight medicinal plants from Western Nepal. Journal of Traditional Chinese Medicine. 2014; 34(5): 584-590.
4.    Naidu N, Sudheer Kumar G, Sivakrishna K, Anjinaik K, Praveen Kumar L, Sneha G. Antimicrobial and antioxidant evolution of aqueous extract of Terminalia chebula using disc diffusion, H2O2 scavenging methods. Asian J. Res. Pharm. Sci. 2017; 7(2): 112-114. doi: 10.5958/2231-5659.2017.00017.0
5.    Kintzios S, Papageorgiou K, Yiakoumettis I, Baricevic D and Kusar A. Evaluation of the antioxidants activities of four Slovene medicinal plant species by traditional and novel biosensory assays. Journal of Pharmaceutical and Biomedical Analysis. 2010; 53: 773–776.
6.    Amidia S, Mojabb F, Moghaddamc AB, Tabiba K and Kobarfard F. A simple electrochemical method for the rapid estimation of antioxidant potentials of some selected medicinal plants. Iranian Journal of Pharmaceutical Research. 2012; 11 (1); 117-121.
7.    Magarelli G, Da Silva JG, De Sousa Filho IA, Lopes ISD, Souza De JR, Hoffmann LV and De Castro CSP. Development and validation of a voltammetric method for determination of total phenolic acids in cotton cultivars. Microchemical Journal. 2013; 109: 23–28.
8.    Fysun O, Khorshid S, Rauschnabel J and Langowski H-C. Detection of dairy fouling by cyclic voltammetry and square wave voltammetry. Food Science and Nutrition. 2020; 00: 1–11.
9.    Mohy-ud-dint AZ, Khan M, Ahmad KM and Kashmiri MA. Chemotaxonomic value of alkaloids in Solanum Nigrum complex. Pakistan Journal of Botany. 2010; 42(1): 653-660.
10.    Xiang L, Wang Y, Yi X and He X. Steroidal alkaloid glycosides and phenolics from the immature fruits of Solanum nigrum. Fitoterapia. 2019; 137: 104268.
11.    Sugumar JK and Guha P. Study on the formulation and optimization of functional soup mix of Solanum nigrum leaves. International Journal of Gastronomy and Food Science. 2020; 20: 100208.
12.    Atanu FO, Ebiloma UG and Ajayi EI. A review of the pharmacological aspects of Solanum nigrum Linn, Biotechnology and Molecular Biology Reviews. 2011; 6 (1): 1-8.
13.    Abas F, Lajis NH, Israf DA, Khozirah S and Kalsom YU. Antioxidant and nitric oxide inhibition activities of selected Malay traditional vegetables. Food Chemistry. 2006; 95(4): 566–573.
14.    Wang YH, Xiang LM, Yi XM and He XJ. Potential anti-inflammatory steroidal saponins from the berries of Solanum nigrum L. (European Black Nightshade). Journal of Agricultural and Food Chemistry. 2017; 21: 4262–4272.
15.    Ahmed S. and Shakeel F. Voltammetric determination of antioxidant character in Berberis lycium Royel, Zanthoxylum armatum and Morus nigra Linn plants. Pak. J. Pharm. Sci. 2012; 25(3): 501-507.
16.    Singleton VL and Rossi JA. Colorimetry of total phenolics with phosphomolybdic – phosphotungstic acid reagents. American Journal of Enology and Viticulture. 1956; 16: 144-158.
17.    Bahorun T, Aumjaud E, Ramphul H, Rycha M, Luximon-Ramma A, Trotin F and Aruoma OI. Phenolic constituents and antioxidant capacities of Crataegus monogyna (Hawthorn) callus extracts. Nahrung. 2003; 47: 191-198.
18.    Kumaran A and Joel Karunakaran R. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT - Food Sci. Technol. 2007; 40: 344-352.
19.    Rice-evans CA, Miller NJ, Bolwell PG, Bramley PM and Pridham JB. The relative antioxidant activities of plant-derived Polyphenolic flavonoids. Free Radical Research. 1995; 22: 375–383.
20.    Rekha Rajendran, Saleem Basha N, Ruby S. Evaluation of In Vitro Antioxidant Activity of stem-bark and stem-wood of Premna serratifolia Lin., (Verbenaceae). Research J. Pharmacognosy and Phytochemistry 2009; 1(1):11-14.
21.    Villano D, Fernandez-Pachon MS, Moya ML, Troncoso AM and Garcıa-Parrilla MC. Radical scavenging ability of polyphe-nolic compounds towards DPPH free radical, Talanta. 2007; 71: 230.
22.    Benzie IF and Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Analytical Biochemistry. 1996; 239: 70-76.
23.    Shubashini K. Sripathi, Subashree, Lalitha P. Electrochemical Behavior of the Aqueous Fraction of the Ethanol Extract of Pisonia grandis (R.Br) at Glassy Carbon Electrode. Asian J. Research Chem. 2011; 4(5): 775-778.
24.    Keffous F, Belboukhari N, Sekkoum K, Djeradi H, Cheriti A and Aboul-Enein HY. Determination of the antioxidant activity of Limoniastrum feei aqueous extract by chemical and electrochemical methods. Cogent Chemistry. 2016; 2: 1186141.
25.    Silva CPD, Sousa MSB, Siguemoto ÉS, Soares RAM and Arêas JAG. Chemical composition and antioxidant activity of jatobá-do-cerrado (Hymenaea stigonocarpa Mart.) flour. Food Science and Technology. 2014; 34(3): 597-603.
26.    Akash Garg, Sanjeev Kumar Mittal. Free radical scavenging, antioxidant activity and phenolic content of Salvadora oleoides Decne Leaves. Res. J. Pharmacognosy and Phytochem. 2018; 10(1): 27-35. doi: 10.5958/0975-4385.2018.00005.5
27.    Liu Y, Chen Y, Wang Y, Chen J, Huang Y, Yan Y, Li L, Li Z, Ren Y and Xiao Y. Total phenolics, capsaicinoids, antioxidant activity, and α-glucosidase inhibitory activity of three varieties of pepper seeds.  International journal of food properties. 2020; 23(1): 1016–1035.
28.    Babaa SA and Malik SA. Determination of total phenolic and flavonoid content, antimicrobialand antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah university for science. 2015; 9: 449-454.
29.    Nidhiya ISR, Pratima A. Tatke, Satish Y. Gabhe, Ashok B. Vaidya. Comparative Antioxidant Activity of Three Garlic Varieties: An in vitro Study. Research J. Pharmacognosy and Phytochemistry 2011; 3(4): 162-165.
30.    Saad-Allah KM and Elhaak MA. Hyperaccumulation activity and metabolic responses of Solanum nigrum in two differentially polluted growth habitats. Journal of the Saudi Society of Agricultural Sciences. 2017; 16: 227–235.
31.    Xianga L, Wanga Y, Yia X and Hea X. Steroidal alkaloid glycosides and phenolics from the immature fruits of Solanum nigrum. Fitoterapia. 2019; 137: 104268.
32.    Andarwulan N, Batari R, Sandrasari DA, Bolling B and Wijaya H. Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chem. 2010; 121 (4): 1231-1235.
33.    Tapas AR, Sakarkar DM and Kakde RB. Flavonoids as nutraceuticals: A Review. Tropical Journal of Pharmaceutical Research. 2008; 7(3): 1089-1099.
34.    Salomi S., Muthukumaran P., Umamaheshwari R. In-vitro antioxidant activity of Azima tetracantha leaves. Research J. Science and Tech. 2012; 4(4): 148-151.
35.    Mohammedi Zohra, Atik Fawzia. Chemical Composition and Antioxidant Activity of Essential Oil from Smyrnium olusatrum L. Asian J. Research Chem. 2011; 4(2): 217-220.
36.    Boulekbache-Makhlouf L, Medouni L, Medouni-Adrar S, Arkoub L and Madani K. Effect of solvents extraction on phenolic content and antioxidant activity of the byproduct of eggplant. Industrial Crops and Products. 2013; 49: 668-674.
37.    Novaes P, Torres PB, Cornub TA, De Carvalho Lopes J, Ferreira MJP and Dos Santos DYAC. Comparing antioxidant activities of flavonols from Annona coriacea by four approaches. South African Journal of Botany. 2019; 123: 253-258.
38.    Lalitha P, Sivakamasundari S. Effect of Variation of Concentration and pH on the Cyclic Voltammetric Behaviour of 4-Methyl-3-Vinyl Quinoline-2(1h)-One at Glassy Carbon Electrode. Asian J. Research Chem. 2010; 3(4): 1015-1019.
39.    Fysun O, Khorshid S, Rauschnabel J and Langowski H-C. Detection of dairy fouling by cyclic voltammetry and square wave voltammetry. Food Sci. Nutr. 2020; 00: 1–11.

Recomonded Articles:

Author(s): Vatchavai Bhaskara Raju, Bonthu Mohan Gandhi, Kamatham Srinivas Sumanth, Kolli Srinivas, Tupakula N Venkata Lakshmi Neeraja

DOI: 10.5958/0974-4150.2017.00030.X         Access: Open Access Read More

Author(s): Kadhiravansivasamy, S. Sivajiganesan, T. Periyathambi, V. Nandhakumar, M. Pugazhenthi

DOI: 10.5958/0974-4150.2017.00016.5         Access: Open Access Read More

Author(s): Gangadhara MM, Dinesha R, Satish Kumar Murari

DOI: 10.5958/0974-4150.2017.00020.7         Access: Open Access Read More

Author(s): Balakrishnan N, Panda A B, Raj N R, Shrivastava A, Prathani R

DOI:         Access: Open Access Read More

Author(s): Anitha Jebamalai Raj, Sudarsanam Dorairaj

DOI:         Access: Open Access Read More

Author(s): VB Kshirsagar, UA Deokate, VB Bharkad, SS Khadabadi

DOI:         Access: Open Access Read More

Author(s): Gopalasatheeskumar K, Ariharasiva Kumar G, Sengottuvel T, Sanish Devan V, Srividhya V

DOI: 10.5958/0974-4150.2019.00062.2         Access: Closed Access Read More

Author(s): Ravi Pratap Pulla, B.S. Sastry, Y. Rajendra Prasad, N. Appala Raju

DOI:         Access: Open Access Read More

Author(s): S Kalidas, B Kameswari, P Devi, B Madhumitha, R Meera, NJ Merlin

DOI:         Access: Open Access Read More

Author(s): Vijay N. Bhosale, Gopinath S. Khansole, Jaman A. Angulwar, Sunil S. Choudhare, Ashok R. Karad, Navanand B. Wadwale

DOI: 10.5958/0974-4150.2017.00126.2         Access: Open Access Read More

Author(s): Tentu Nageswara Rao, Botsa Parvatamma, Karri Apparao, Jakka Mastan

DOI: 10.5958/0974-4150.2017.00059.1         Access: Open Access Read More

Author(s): Shlini P., Siddalinga Murthy K.R.

DOI:         Access: Open Access Read More

Author(s): K. Balamuralikrishna, B. Syamasundar

DOI:         Access: Open Access Read More

Author(s): Cijo John, Mohd Idris, P. Sudhakar, Priyankar Ghosh, K.M. Varshney, S.K. Shukla

DOI:         Access: Open Access Read More

Author(s): P Nagaraju, K Krishnachaithanya, VDN Srinivas, SVN Padma

DOI:         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles


Recent Articles




Tags