Author(s):
Mahamadi Ouedraogo, Dominique Saga Kaboré, Benjamin Bazié, Remy K Bationo, Moumouni Koala, Constantin M Dabiré, Eloi Palé, Mouhoussine Nacro
Email(s):
mahaouedraogo@yahoo.fr
DOI:
10.52711/0974-4150.2024.00055 
Address:
Mahamadi Ouedraogo1,2*, Dominique Saga Kaboré1, Benjamin Bazié1,3, Remy K Bationo1,4, Moumouni Koala1,5, Constantin M Dabiré1,6, Eloi Palé1, Mouhoussine Nacro1
1Laboratoire de Chimie Organique et Physique Appliquées, Département de Chimie, UFR-SEA, Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
2Université Norbert ZONGO, avce Maurice Yameogo, Koudougou BP 376, Burkina Faso.
3Ecole Polytechnique de Ouagadougou, Ouagadougou, Burkina Faso.
4CNRST/IRSAT, Département Substances Naturelles, 03 BP 7047 Ouagadougou 03 Burkina Faso.
5Laboratoire de Recherche-Développement de Phytomédicaments et Médicaments/Institut de Recherche en Science de la Santé (IRSS/CNRST), 03 BP 7047 Ouagadougou 03, Burkina Faso.
6Laboratoire de Chimie et Energie Renouvelables, Université Nazi BONI, 01 BP 1091 Bobo 01, Burkina Faso.
*Corresponding Author
Published In:
Volume - 17,
Issue - 6,
Year - 2024
ABSTRACT:
Carotenoids represent a family of compounds found in plant foods. They are fascinated by the conjunction of their biological properties in preventing diseases such as atherosclerosis, cancers, and premature aging. Mung bean is a legume of the Vigna genus whose seeds are rich in bioactive compounds, particularly carotenoids. This work aimed to identify the most carotenoids in the seeds of mung beans acclimated to Burkina Faso by HPLC-MS/MS. To achieve this, the seeds were extracted with a cold acetone/hexane mixture (50/50, V/V). After saponification, the crude extract was characterized by thin layer chromatography (TLC) and UV-visible spectrometry. Test methods using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS) made it possible to identify the majority carotenoids in the extracts. The thin layer chromatographic profile of the crude extract revealed two spots with Rf= 0.48 and 0.87 corresponding respectively to xanthophylls and carotenes. The UV-visible spectra of the spots each showed an absorption maximum of 450nm. HPLC-MS/MS analysis identified the structures of three carotenoids: ß-cryptoxanthin, a-tocopherol, and ß-carotene. These compounds identified in V. radiata seeds are very useful in many fields, including pharmaceutical, agri-food and biological. The HPLC-MS/MS technique represents one of the useful and efficient tools for the identification of bioactive substances in crude extracts.
Cite this article:
Mahamadi Ouedraogo, Dominique Saga Kaboré, Benjamin Bazié, Remy K Bationo, Moumouni Koala, Constantin M Dabiré, Eloi Palé, Mouhoussine Nacro. Carotenoid profiling by HPLC-MS/MS of Mung bean (Vigna radiata (L) R. Wilczek 1854), acclimated to Burkina Faso. Asian Journal of Research in Chemistry.2024; 17(6):324-0. doi: 10.52711/0974-4150.2024.00055
Cite(Electronic):
Mahamadi Ouedraogo, Dominique Saga Kaboré, Benjamin Bazié, Remy K Bationo, Moumouni Koala, Constantin M Dabiré, Eloi Palé, Mouhoussine Nacro. Carotenoid profiling by HPLC-MS/MS of Mung bean (Vigna radiata (L) R. Wilczek 1854), acclimated to Burkina Faso. Asian Journal of Research in Chemistry.2024; 17(6):324-0. doi: 10.52711/0974-4150.2024.00055 Available on: https://ajrconline.org/AbstractView.aspx?PID=2024-17-6-3
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