Author(s):
A. Habchi, M. Dekmouche, C. Hamia, M. Saidi, M. Yousfi, A. Bouguerra
Email(s):
amina.bouga25@gmail.com
DOI:
10.52711/0974-4150.2021.00030 
Address:
A. Habchi1, M. Dekmouche1, C. Hamia2, M. Saidi1, M. Yousfi2, A. Bouguerra*
1Univ Ouargla, Fac. Des Mathématiques et Des Sciences de la Matiere, Lab. Valorisation et Promotion des Ressources Sahariennes (VPRS), Route de GhardaIa, Ouargla, Algeria.
2Univ. Laghouat, fac. des mathématiques et des sciences de la matière, Lab. valorisation et promotion des ressources sahariennes (VPRS), Route de Ghardaïa, Ouargla, Algeria.
*Corresponding Author
Published In:
Volume - 14,
Issue - 3,
Year - 2021
ABSTRACT:
This study was designed to evaluate the antioxidant activity of phenolic compounds for six cultivars of date from In Saleh region (Phoenix dactylifera L.), which were extracted by reflux with solvents, 80% methanol and 70% aceton after the phenolic and flavonoid content were estimated. The total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were measured using Folin–Ciocalteau and aluminum chloride colorimetric methods, respectively. Antioxidant activity acetone/H2O and methanol/H2O extracts using ABTS, DPPH, FRAP, TAC and CUPRAC assays. The results showed that methanolic solvent reflux gives better yields. The highest level of phenolic compounds for all dates varieties was found in the 80% methanol extract. This level ranged from 8.04 ± 0.14 to 46.81 ± 1.22 mg GAE/100 g fresh weight and flavonoid content was observed relatively low for both two solvents. However, extraction into 70% acetone gave the highest antioxidant activity with all assay. These results showed that dates had strongly scavenging activity on DPPH reaching 3.12 µg/mL in methanol/H2O from Cul1 (6.9 µg/mL with ascorbic acid), also on ABTS reaching 6.51 µg/mL in acetone/H2O from Cul2 (104.98 µg/mL with ascorbic acid).
Cite this article:
A. Habchi, M. Dekmouche, C. Hamia, M. Saidi, M. Yousfi, A. Bouguerra. Extraction of Phenolic Compounds of six Algerian date (Phoenix dactylifera L.) cultivars from Ain-Saleh region, using Reflux method and Screening of Antioxidant Activity in vitro. Asian Journal of Research in Chemistry. 2021; 14(3):161-7. doi: 10.52711/0974-4150.2021.00030
Cite(Electronic):
A. Habchi, M. Dekmouche, C. Hamia, M. Saidi, M. Yousfi, A. Bouguerra. Extraction of Phenolic Compounds of six Algerian date (Phoenix dactylifera L.) cultivars from Ain-Saleh region, using Reflux method and Screening of Antioxidant Activity in vitro. Asian Journal of Research in Chemistry. 2021; 14(3):161-7. doi: 10.52711/0974-4150.2021.00030 Available on: https://ajrconline.org/AbstractView.aspx?PID=2021-14-3-2
REFERENCES:
1. Ghiaba, Z., Boukouada, M., Djeridane, A., Saidi, M.; Yousfi, M., 2012: Screening of Antioxidant Activity and Phenolic Compounds of Various Date Palm (Phoenix Dactylifera) Fruits From Algeria. Mediter J Nutr Metab. 5: 119-126.
2. Mansouri, A., Embarek, G., Kokkalou, E., and Kefalas, P. (2005). Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera). Food Chemistry, 89, 411–420.
1. 3.FAO. Statistical Databases (2013). www.FAO.org
3. Al-Hooti, S., Sidhu, J., and Quabazard, H. (1995). Studies on the physicochemical characteristics of date fruits of five UAE cultivars at different stages of maturity. Arab Gulf Journal of Scientific Research, 13, 553–569.
6. Al-Shahib, W., and Marshal, R. J. (2003). The fruit of the date palm: Its possible use as the best food for the future. International Journal of the Food Science and Nutrition, 54, 247–259
7. Sawaya, W. N., Khatchadourian, H. A., Khalil, J. K., Safi, W. M., and Alshalhat, A. (1982). Growth and compositional changes during the various developmental stages of some Saudi Arabian date cultivars. Journal of Food Science, 47, 1489–1493.
8. M. Al-Farsi, C. Alasalvar, M. Al-Abid, K. Al-Shoaily, M. Al-Amry, and F. Al-Rawahy, “Compositional and functional characteristics of dates, syrups, and their by-products,” Food Chemistry, vol. 104, no. 3, pp. 943–947, 2007.
9. M. Al-Farsi, A. Morris, and M. Baron, “Functional properties of omani dates (Phoenix dactylifera L.),” Acta Horticulturae, vol. 736, pp. 479–487, 2007
10. Regnault-Roger, C., Hadidane, R., Biard, J. F., and Boukef, K. (1987). High performance liquid and thin-layer chromatography determination of phenolic acids in date palm (Phoenix dactylifera) products. Food Chemistry, 25, 61–71.
11. Al-Farsi, M., Alasalvar, C., Morris, A., Baron, M. and Shaihdi, F. (2005). Comparison of antioxidant activity, anthocyanins, carotenods, and phenolics of three native fresh and sun-dried date (Phoenix dactylifera. L.) varieties grown in Oman. Journal of Agricultural and Food Chemistry, 53, 7592–7599.
12. Fridovich, I. (1978). The biology of oxygen radicals. Science, 201, 875–880.
13. Kinsella, J. E., Frankel, E., German, B., and Kanner, J. (1993). Possible mechanisms for the protective role of antioxidants in wine and plant foods. Food Technology, 47, 85–89.
14. Kaur, C. and Kapoor ,H.C. (2001). Antioxidants in fruit and vegetables- The millennium’ health. International J. Food Sci. Tech., 36: 703-725.
15. Langseth L, Oxidants, antioxidants, and disease prevention: ILSI Europe; 1995.
16. Naczk M, Amarowicz R, Zadernowski R, Pegg RB, Shahidi F. Antioxidant activity of crude phenolic extracts from wild blueberry leaves. Pol J Food Nutr Sci. 2003;12(53):166-190.
17. Caillet.S.;Salmieri,S. and Lacroix,M.(2006). Evaluation of free radical –scavenging properties of commercial grape phenol extract by a fast colometris method. Food Chem., 95:1-6.
18. Balsano C, Alisi A. Antioxidant effects of natural bioactive compounds. Curr. Pharm. Des. 15; 2009: 3063– 3073.
19. Divya Nambath et al. Free Radical Scavenging Activity of the Methanol Extract of Heracleam Candolleanum (Wight-Et-Arn), Indo American Journal of Pharm Research. 2014:4(05),2491-2495.
20. Kulkarni, A.P., Aradhya, S.M., Divakar, S., 2004. Isolation and identification of radical scavenging antioxidant – punicalagin from the pith and carpellary membrane of pomegranate fruit. Food Chem. 87, 551–557.
21. Benmeddour, Z., Mehinagic, E., Le Meurlay, D., Louaileche, H., 2013. Phenolic composition and antioxidant capacities of ten Algerian date (Phoenix dactylifera L.) cultivars: A comparative study.Journal of functional foods. 5: 346-354.
22. W.Kchaou, F. Abbès, C. Blecker, H Attia, S Besbes. Effects of extraction solvents on phenolic contents and antioxidant activities of Tunisian date varieties (Phoenix dactylifera L.). Industrial crops and products 45 (2013) 262- 269.
23. Vinson, J.A., Zubik, L., Bose, P., Samman, N. and Proch, J. (2005). Dried fruits: excellent in vitro and in vivo antioxidants. Journal of the American College of Nutrition, 24, 44–50.
24. Collin S. and Jean Crouzet, (2011). "Polyphenols and processes". Edition Lavoisier. P. 337.
25. Falleh H., Ksouri R., Chaieb K., Karray-Bouraoui N., Trabelsi N., Boulaaba Mr., Abdelly C., 2008- phenolic composition of Cynaracardunculus L. organs, and their biological activities, C. R. biologies. Vol. (331). 372-379.
26. Slinkard K, Singleton VL. 1977. Total phenol analyses, Automation and comparison with manual methods. Am J Enol Viticulture 28, 49-55.
27. Chang CC, Yang MH, Wen HM, Chern JC. 2002. Estimation of total flavonoids content in Propolis by two complementary colorimetric methods. J Food Drug Anal 10,178-182.
28. Djeridane A.,Yousfi Mr., Nadjemi B., Boutassouna D., store, P. and Vidal N.; antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds, Food Chemistry, 654-660 (2006)
29. Molyneux P. 2004. Use of DPPH to testing antioxidant activity. J. Sci. Technol 26(2), 212-219.
30. Gadow JE and Hansmann CF. Comparison of antioxidant activity of aspulathin with that of other plant phenols of Rooiboos Tea (Asphalathus linearis), a-tocopherol BHT, and BHA. J Agric Food Chem. 1997; 832-838.
31. Popovici C., Saykova I. And Tylkowski B., (2009). "Evaluation of the activity of antioxidant phenolic compounds by the reactivity with the free radical DPPH". Review of Industrial Engineering 2009, 4, 25-39.
32. Liu, T. Z.; Chin, N.; Kiser, M. D.; Bigler, W. N. Specific spectrophotmetry of ascorbic acid in serum or plasma by use of ascorbate oxidase. Clin. Chem. 1982, 28, 2225–2228.
33. Benzie, I. F. F. An automated, specific, spectrophotometric method for measuring ascorbic acid in plasma (EFTSA). Clin. Biochem. 1996, 29, 111–116.
34. Benzie, I. F. F. and J. J. strain (1996). "The ferric reducing ability of plasma (FRAP) as a measure of 'antioxidant power': The FRAP assay." Analytical Biochemistry 239(1): 70-76.
35. R. Pulido, L. Bravo, F. Saura-calixto, Antioxidant activity of dietary poly-phenols as determined by a modified ferric reducing/antioxidant power assay, Journal of Agricultural Food Chemistry. 48 (2000) 3396-3402
36. Shirwaikar A and Govindraju R, et al. Anti-inflammatory activity Lam. Biol Pharm Bull.26; 2003; 1424.
37. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic BioMed. 1999; 26: 1231-37.
38. Cai, Y., Luo, Q., Sun, M. and Corke, H. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Science 74: 2157-2184.
39. Apak, R., Güçlu, K., Özurek, M., and Karademir, S. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of Neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry 52(26), 7970, 7981
40. K. Sowndhararajan, S.C. Kang, Protective effect of ethyl acetate fraction of Acacia ferruginea DC. against ethanol-induced gastric ulcer in rats. Journal of Ethnopharmacology, 148 (1), (2013) 175–181.
41. Sulaiman, S.F., Sajak, A.A.B., Ooi, K.L., Supriatno Seow, E.M., 2011. Effect of solvents in extracting polyphenols and antioxidants of selected raw vegetables. J. Food Compost. Anal. 24, 506–515.
42. S. Jokić, D. Velić, Mr. Bilić, A. Bucić-Kojić, Mr. inićand Plan S.Tomas. Modeling of the process of Solid-Liquid extraction of total polyphenols from soybeans. J. Food Sci. Vol. 28. (2010). pp. 206-212.
43. Singh, A. P., Luthria, D., Wilson, T., Vorsa, N., Singh, V., Banuelos, G. S. and Pasakdee, S. (2009). Polyphenols content and antioxidant capacity of eggplant pulp. Food Chemistry 114, 955-961.
44. Raigón, Mr. D., PROHENS, J., Julio E. Muñoz-Falcón and Nuez, F. (2008). Comparison of eggplant landraces and commercial varieties for fruit content of phenolics, minerals, dry matter and protein. Journal of Food Composition and Analysis 21, 370- 376.
45. F. M. Al-Jasass, M. Siddiq, and D. S. Sogi (2015). Antioxidants Activity and Color Evaluation of Date Fruit of Selected Cultivars Commercially Available in the United States. Hindawi Publishing Corporation, Vol. (15), 1–5.
46. Rice-Evans, C. A., Miller, N. M., and Paganda, G. 1996: Structureantioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine,20: 93-956.
47. Jayaprakasha, G.K., Singh, R.P., Sakariah, K.K., 2001. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem. 73, 285–290.
48. Moure, A., Cruz, J.M., Franco, D., Dominguez, J.M., Sineiro, J., Dominguez, H., Núnez, M.J., Parajó, J.C., 2001. Natural antioxidants from residual sources. Food Chem. 72, 145–171.
49. Sobhy, M., Abdalla, S.M.A., 2009. Total phenolic contents and antioxidant activity of corn tassel extracts. Food Chem. 112, 595–598.