Author(s):
Chandramohan Marimuthu, Jayaganesh Murugan, Sruthi Sukumar, Ahamadabdul Quadir Buhari Khaleel
Email(s):
info@microcoreresearch.com
DOI:
10.52711/0974-4150.2023.00024
Address:
Chandramohan Marimuthu*, Jayaganesh Murugan, Sruthi Sukumar, Ahamadabdul Quadir Buhari Khaleel
Microcore Research Laboratories India Pvt Ltd., 9th km, 30 Feet Road, 204 – A Poondurai Main Road,
Checkmedu, Erode - 638115, Tamil Nadu.
*Corresponding Author
Published In:
Volume - 16,
Issue - 2,
Year - 2023
ABSTRACT:
The main objective of this study relates to simple and economic process with stepwise enzymes treatment for the cost effective production of ethanol from Bagasse waste and Egg albumin waste. Sugarcane Bagasse waste was selected as inexpensive cellulosic feed stock, which has the general composition of 40-45% cellulose, 28-10% hemicellulose, and 19-21% lignin. Removal of lignin, tannin, to lose the bagasse fiber matrix by chemical treatment and stage wise addition of neutral and acid cellulose to facilitate release of reducing sugars by enzymatic treatment. Apparently the production of inexpensive sterile organic nitrogen from egg albumin waste with neutral protease and peptidase by enzymatic process. The obtained C: N was mixed in the ratio of 3:1used for the cost effective production of Ethanol from Saccharomyces cerevisiae. Further this study includes the unhydrolysed part of Bagasse processing for the production of insoluble cellulose fiber as dietary supplement. 50 % reducing sugars recovered from Sugarcane Bagasse by chemical-enzyme coupled treatment and 90 % free amino acids recovered from albumin protein by enzyme treatment. 3:1 ratio of Carbohydrate: Nitrogen mixed in definite proportion inoculated with yeast enhances the conversion of ethanol to 79g/L yield on batch mode.
Cite this article:
Chandramohan Marimuthu, Jayaganesh Murugan, Sruthi Sukumar, Ahamadabdul Quadir Buhari Khaleel. Shake Flask Studies on Ethanol Production from Hydrolyze Bagasse and Egg Albumin Waste by using Enzymes and Chemicals. Asian Journal of Research in Chemistry 2023; 16(2):149-4. doi: 10.52711/0974-4150.2023.00024
Cite(Electronic):
Chandramohan Marimuthu, Jayaganesh Murugan, Sruthi Sukumar, Ahamadabdul Quadir Buhari Khaleel. Shake Flask Studies on Ethanol Production from Hydrolyze Bagasse and Egg Albumin Waste by using Enzymes and Chemicals. Asian Journal of Research in Chemistry 2023; 16(2):149-4. doi: 10.52711/0974-4150.2023.00024 Available on: https://ajrconline.org/AbstractView.aspx?PID=2023-16-2-8
REFERENCES:
1. Memon A A, Shah F A, Kumar N (2003). Bioethanol Production from Waste Potatoes as a Sustainable Waste-to-energy Resource via Enzymatic Hydrolysis. International Conference on Sustainable Energy Engineering. pp.81-89.
2. Balat M,Balat H, Oz C (2008). Progress in bioethanol process in. Progr. Energy Combust. Sci. vol-34(5), pp. 551–573.
3. Hahn-Hagerdal B, Galbe M, Gorwa-Grauslund M F., Liden G, Zacchi G (2006). Bio-ethanol – the fuel of tomorrow from the residues of today. Trends Biotechnol. vol-24(12), pp.549-556.
4. Gupta A, Verma J P (2015). Sustainable bio-ethanol production from agro-residues: A review. Renewable Sustainable Energy Rev. vol- 41, pp. 550-567.
5. Gruno M, Vaeljamaee P, Pettersson G, Johansson G (2004). Inhibition of the Trichodermareesei cellulases by cellobiose is strongly dependent on the nature of the substrate. Biotechnol Bioengin. vol-86, pp.503–511.
6. Cheng K and Zhang J (2006) . (447m) Ethanol from Sugar Cane Bagasse by a Simultaneous Saccharification and Fermentation Process (Ssf) with Candida Krusei Icm-Y-05. Green Biotechnology poster session.-(15008).
7. Rezende C A., De Lima M, Maziero P, Deazevedo E, Garcia W and Polikarpov I (2011). Chemical and morphological characterization of sugarcane bagasse submitted to a delignification process for enhanced enzymatic digestibility. Biotechnology for Biofuels. vol. 4, article 54.
8. Ime I M (2010). Biochar from anaerobically digested sugarcane bagasse. in Graduate School, University of Florida.
9. Pandey A, Soccol C R S., Nigam P and Soccol V T (2000). Biotechnological potential of agro-industrial residues. I: sugarcane bagasse. Bioresource Technology. vol. 74 (1), pp. 69–80.
10. Baeta B E L., Lima D R S., Adarme O F H., Gurgel L V A and Aquino S F D (2016). Optimization of sugarcane bagasse autohydrolysis for methane production from hemicellulose hydrolyzates in a biorefinery concept. Bioresource Technology. vol-200, pp. 137–146.
11. Kim TH and Lee YY (2007). Pretreatment of corn stover by soaking in aqueous ammonia at moderate temperatures. Appl Biochem Biotechnol. pp.81–92.
12. Chiaramonti D, Prussi M, Ferrero S, Oriani L, Ottonello P, Torre P,Cherchi F (2012). Review of pretreatment processes for lignocellulosic ethanol production, and development of an innovative method. Biomass Bioenergy. vol-46, pp.25-35
13. Silveira, M H L., Morais A R C., Lopes A M C., Olekszyszen D N., Bogel-Lukasik R, Andreaus J, Ramos L P (2015). Current Pretreatment Technologies for the Development of Cellulosic Ethanol and Biorefineries. Chem Sus Chem. vol- 8(20),pp. 3366-3390.
14. Ramos L P (2003), Quim. Nova vol-26,pp.863
15. Mosier N, Wyman C, Dale B, Elander R, Lee YY., Holtzapple M, Ladisch M (2005). Features of promising technologies for pretreatment of lignocellulosic biomass. BioresTechnol. vol-96, pp.673–686
16. rreli N, Agelli M, Pisu B, Rescigno A, Sanjust E, Rinaldi A(2002). Complete and efficient enzymic hydrolysis of pretreated wheat straw. Proc Biochemol. 37, pp.937–941.
17. Ingram LO., Conway T, Clark DP., Sewell GW., Preston JF (1987). Genetic engineering of ethanol production in Escherichia coli. Appl Environ Microbiol. vol-53, pp.2420–2425.
18. Matthew H, Ashley O, Brian K, Alisa E, Benjamin JS (2005). Wine making 101.
19. Millichip RJ and Doelle H W (1989). Large-scale ethanol production from Milo Sorghum using Zymomonasmobilis. Proc Biochem. vol-24, pp.141–145.
20. Saddler J.N and Chan M.K.H (1982). Optimization of Clostridium thermocellum growth on cellulose and pretreated wood substrates. European J Appl Microbiol Biotechnol. vol-16, pp.99–104.
21. Gong CS, Maun CM, Tsao G T (1981). Direct fermentation of cellulose to ethanol by a cellulolytic filamentous fungus Monilia sp. Biotechnol Lett. vol-3, pp.77–82.
22. Yamauchi H, Akita O, Obata T, Amachi T, Hara S (1989). Production and application of a fruity odor in a solid state culture of Neurospora sp. using pregelatinized polish rice. Agric Biol Chem. vol-53, pp.2881–2888.
23. Pastore GM., Park YK., Min DB (1994). Production of a fruity aroma by Neurospora from beiju. Mycol Res. vol-98 pp.25–35.
24. Sugawara E, Hashimoto S, Sakurai Y, Kobayashi A (1994). Formation by yeast of the HEMF 4-hydrpxy-2 or 5.-ethyl-5 or 2-methyl-3 2H.-furanone aroma components in Miso with aging. Biosci Biotechnol Biochem. vol-58, pp.1134–1135.
25. Ito K, Yoshida K, Ishikawa T, Kobayashi S (1990). Volatile compounds produced by fungus Aspergillusoryzae in rice koji and their changes during cultivation. J Ferment Bioengin. vol-70, pp.169–172.
26. Gervais P and Sarrette M (1990). Influence of age of mycelia and water activity on aroma production by Trichodermaviride. J Ferment Bioengin. vol- 69, pp.46–50.
27. APHA (1999). Standard Methods for the Examination of Water and Wastewater. American Public Health Association, American Water Works Association, Water Environment Federation, Washington, DC, USA.
28. Miller G L (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry. vol-31(3), pp.426–428.
29. Talha Z, Ding W, Mehryar E, Hassan M and Bi J (2016). Alkaline Pretreatment of Sugarcane Bagasse and Filter Mud Codigested to Improve Biomethane Production. BioMed Research International. pp.1-10.
30. Michalska K, Bizukojc M and Ledakowicz S (2015). Pretreatment of energy crops with sodium hydroxide and cellulolytic enzymes to increase biogas production. Biomass and Bioenergy. vol. 80, pp. 213–221.
31. Modenbach A A and Nokes S (2014). Effects of sodium hydroxide pretreatment on structural components of biomass. American Society of Agricultural and Biological Engineers (ASABE). vol. 57, no. 4, pp. 1187–1198.