Nilesh Patel, Shailesh Shah, Ishwarsinh Rathod, Dhaval Patel, Biraju Patel
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Nilesh Patel1*, Shailesh Shah2, Ishwarsinh Rathod3, Dhaval Patel4 and Biraju Patel4
1Department of Pharmaceutical Sciences, Saurashtra University, Rajkot-360005, Gujarat, India
2Department of Quality Assurance, Maliba pharmacy College, Bardoli, Gujarat, India
3Department of Quality Assurance, L.M. College of Pharmacy, Ahmedabad-380009, Gujarat, India
4Veerayatan Institute of Pharmacy, Mandvi-370460, Gujarat, India
Volume - 3,
Issue - 2,
Year - 2010
A simple, specific and precise HPTLC method was developed and validated for estimation of aceclofenac in photodegraded sample. The method involves separation of sample and photodegradation product on TLC aluminum sheets pre-coated with silica gel 60 F254 plate using a mixture of Toluene: Ethyl acetate: Glacial acetic acid: Methanol (6.00:1.00:0.20:0.15 v/v/v/v) as the mobile phase. Detection of the spot was carried out at 284nm in absorbance/reflectance mode. The retardation factor (Rf) for aceclofenac was found to be 0.35±0.03. The linearity range was found to be 50-150ng/spot with r2= 0.9931. The validated method was applied for determination of photodegradation kinetic study. Photodegradation reaction at various experimental conditions follows zero order kinetic and rate constant (K) was determined to be 0.4855 ng µl-1 min-1. T1/2 and T90% was found to be 116.78 min-1 and 23.35 min-1 respectively.
Cite this article:
Nilesh Patel, Shailesh Shah, Ishwarsinh Rathod, Dhaval Patel, Biraju Patel. Photodegradation Kinetic Study of Aceclofenac by HPTLC Method. Asian J. Research Chem. 3(2): April- June 2010; Page 500-503.