ISSN

0974-4150 (Online)
0974-4169 (Print)


Author(s): K. Mahendra, Y.L. N.Murthy, C.V. Narasimha Rao, K. Bala Murali Krishna

Email(s): kolisetti_m@yahoo.com

DOI: Not Available

Address: K. Mahendra1*, Y.L. N.Murthy2, C.V. Narasimha Rao3 and K. Bala Murali Krishna4
1Sr.Executive(R&D), M/s.Bio-Pharma Laboratories Pvt.Ltd.,Guntur-522002, Andhra Pradesh, India
2Professor and Head, Department of Organic Chemistry, Andhra University, Visakhapatnam-530003, A. P., India
3Principal Scientist, Division of Crop Chemistry and Soil Science, Central Tobacco Research Institute, Rajahmundry-533105, Andhra Pradesh, India
4Faculty, Department of chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, A. P., India.
*Corresponding Author

Published In:   Volume - 4,      Issue - 7,     Year - 2011


ABSTRACT:
A rapid and sensitive liquid chromatography–mass spectrometry (LC–MS/MS) method with multiple reaction monitoring (MRM) was developed for the determination of solanesol in tobacco. Sample preparation was performed by ultrasonic extraction with methanol and then supernatant was extracted with hexane. The method used atmospheric pressure chemical ionization (APCI) detection in positive-ion mode. The separation of solanesol was performed on a Symmetry RP18 column with a mixture of acetonitrile and isopropanol (1:1, v/v) containing 2mM ammonium acetate as mobile phase. Quantification of solanesol was performed by the standard addition method. The limit of quantification (LOQ) and limit of detection (LOD) of solanesol were, respectively, 5.0 ng/ml (S/N = 10) and 1.5 ng/ml (S/N = 3). The relative standard deviations of peak area were 0.89 and 1.12% for intra-day and inter-day, respectively. The recovery of solanesol ranged from 97.72 to 99.67% and the corresponding R.S.D.s were less than 2.7%. Analysis took 5 min, making the method suitable for rapid determination of solanesol in tobacco. The proposed method has been successfully applied to the analysis of solanesol in various tobacco samples.


Cite this article:
K. Mahendra, Y.L. N.Murthy, C.V. Narasimha Rao, K. Bala Murali Krishna.Quantitative Determination of Solanesol in Tobacco by Liquid Chromatography–Mass Spectrometry (LC-MS). Asian J. Research Chem. 4(7): July, 2011; Page 1125-1130.

Cite(Electronic):
K. Mahendra, Y.L. N.Murthy, C.V. Narasimha Rao, K. Bala Murali Krishna.Quantitative Determination of Solanesol in Tobacco by Liquid Chromatography–Mass Spectrometry (LC-MS). Asian J. Research Chem. 4(7): July, 2011; Page 1125-1130.   Available on: https://ajrconline.org/AbstractView.aspx?PID=2011-4-7-21


Recomonded Articles:

Author(s): Vatchavai Bhaskara Raju, Bonthu Mohan Gandhi, Kamatham Srinivas Sumanth, Kolli Srinivas, Tupakula N Venkata Lakshmi Neeraja

DOI: 10.5958/0974-4150.2017.00030.X         Access: Open Access Read More

Author(s): Gopalasatheeskumar K, Ariharasiva Kumar G, Sengottuvel T, Sanish Devan V, Srividhya V

DOI: 10.5958/0974-4150.2019.00062.2         Access: Open Access Read More

Author(s): VB Kshirsagar, UA Deokate, VB Bharkad, SS Khadabadi

DOI:         Access: Open Access Read More

Author(s): Sachin Patil, B Ramesh, AR Hareesh, Kiran Patil, Ashok Dhokane

DOI:         Access: Open Access Read More

Author(s): Ravi Pratap Pulla, B.S. Sastry, Y. Rajendra Prasad, N. Appala Raju

DOI:         Access: Open Access Read More

Author(s): K. Vijaya Sri, S. Sruthi, l.D. Srinivas

DOI:         Access: Open Access Read More

Author(s): Tentu Nageswara Rao, Botsa Parvatamma, Karri Apparao, Jakka Mastan

DOI: 10.5958/0974-4150.2017.00059.1         Access: Open Access Read More

Author(s): P Kalaiselvi, R Vijay Amirtharaj, T Venkatachalam, N Senthil Kumar

DOI:         Access: Open Access Read More

Author(s): M. Yogeshwar Reddy, V. Ramesh, Ch. Kista Reddy, N. Venugopal, G. Saravanana, M.V. Suryanarayana, B. Shyam Sunder , R.Surendranath Reddy, G. Raju

DOI:         Access: Open Access Read More

Author(s): Cijo John, Mohd Idris, P. Sudhakar, Priyankar Ghosh, K.M. Varshney, S.K. Shukla

DOI:         Access: Open Access Read More

Author(s): K. Balamuralikrishna, B. Syamasundar

DOI:         Access: Open Access Read More

Author(s): Ravindra Pandey, Swarnlata Saraf, S. Saraf

DOI:         Access: Open Access Read More

Author(s): R. Anantha Kumar, G. Raveendra Babu, M. Sowjanya, M. Ramayyappa

DOI: 10.52711/0974-4150.2021.00031         Access: Open Access Read More

Author(s): Krishna Veni Nagappan, Meyyanathan S N, Rajinikanth B Raja, Elango Kannan

DOI:         Access: Open Access Read More

Asian Journal of Research in Chemistry (AJRC) is an international, peer-reviewed journal devoted to pure and applied chemistry..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0974-4150 

Popular Articles


Recent Articles




Tags