Vilas A. Arsul, R. O. Ganjiwale, P. G. Yeole
Vilas A. Arsul*1, 2, R. O. Ganjiwale1 and P. G. Yeole1
1P.G. Department of Quality Assurance, Institute of Pharmaceutical Education and Research, Borgaon (Meghe), Wardha-442001, Maharashtra, India
2Dr. Vedprakash Patil Pharmacy College, Aurangabad-431005, Maharashtra, India
Volume - 4,
Issue - 5,
Year - 2011
A simple, precise, accurate, rapid and reproductive HPTLC procedure was developed for development and validation of phyllanthin in tablet dosage form at a single wavelength. The mobile phase used was a combination of Toluene-Ethyl acetate [9:1 V/V]. The detection of the combined dosage form was carried out at 200nm. The Rf value of standard phyllanthin was 0.83 ± 0.04 and matches to the phyllanthin present in polyherbal tablet. The phyllanthin content of tablet was determined and it was found to be 0.178 % w/w ±0.0016.The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.
Cite this article:
Vilas A. Arsul, R. O. Ganjiwale, P. G. Yeole. Development and Validation of Phyllanthin by HPTLC in Hepatoprotective Polyherbal Tablet Dosage Form. Asian J. Research Chem. 4(5): May, 2011; Page 815-817.